Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (29): 5321-5326.doi: 10.3969/j.issn. 2095-4344.2012.29. 001

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Osteoinductivity of chitosan/nano-hydroxyapatite bioelectret barrier membranes

Ao Dan-ting1, Wang Yan-ying2, Wang Ping2, Qu Yi-li1, Man Yi2, Chen Zhi-qing1   

  1. 1State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, Sichuan Province, China;
    2Center of Implantation, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2011-11-25 Revised:2011-12-26 Online:2012-07-15 Published:2012-07-15
  • Contact: Chen Zhi-qing, Professor, Doctoral supervisor, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, Sichuan Province, China chenzhiqing40@ 126.com
  • About author:Ao Dan-ting☆, Studying for doctorate, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, Sichuan Province, China kathy123@sina.com

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Abstract:

BACKGROUND: Currently, the barrier membranes that applied in guided bone regeneration have no osteoinductivity.
OBJECTIVE: To explore the effects of composite bioelectret membranes after polarization on compatibility and activity of osteoblasts.
METHODS: Chitosan/nano-hydroxyapatite bioelectret composite membranes were prepared by grid-controlled corona charge. The surface potential decay of the membranes and the proliferation and differentiation of osteoblasts seeded on them were evaluated.
RESULTS AND CONCLUSION: The surface potential was obviously reduced in 1 day, but the speed of decay slowed gradually during the following 9 days. Compared with the unpolarized group, the osteoblasts on the bioelectret membranes had clearer boundaries and more pseudopodia. Moreover, the proliferation ability and alkaline phosphatase activity of the cells seeded on bioelectret membranes were higher than those of the unpolarized group (P < 0.05). But the direct immunofluorescence showed no significant difference in actin between the two groups. In conclusion, bioelectret mebrane is a new type of barrier membrane with osteoinductivity and can improve the proliferation and differentiation of osteoblasts.

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